Page 40 - Grapevine NovDec 2020
P. 40

Around The Vineyard
               Around The Vineyard















































                      Symptoms of Grapevine red blotch virus – note the red veins in the leaf





               sified in three genera, Ampelovirus, Closterovirus,   placed GRBV in a new genus, named Grablovirus,
               and Velarivirus. Grapevine leafroll associated       within the Geminiviridae family.   As stated above,
               virus -1, GLRaV-3, and GLRaV-4 belong to the         it has been difficult to demonstrate Koch’s postu-
               Ampelovirus genus.  Grapevine leafroll associated    lates, with grapevine-infecting viruses. There are
               virus -2 is a Closterovirus and GLRaV-7 is a member   many reasons for this.  Firstly, there are no alter-
               of the Velarivirus genus.  Some researchers claim    native hosts that are susceptible to most grapevine
               that GLRaV-7 should not be considered a leafroll     infecting viruses (some exceptions exists).
               virus because it only produces mild symptoms in
               grapevines.   Further, recent research has shown       Secondly and most important, grapevine viruses
               that GLRaV-7 was isolated from a mixed leafroll      cannot be mechanically transmitted onto grape-
               infected vine and symptoms were due to the other     vines.  These viruses need to be introduced to a
               leafroll virus present at the time.  When found      vine via grafting (graft-transmission) and/or need
               alone GLRaV-7 does not appear to show typical lea-   a biological vector for successful transmission.   Dr.
               froll symptoms.                                      Marc Fuchs team at Cornell University was able to
                                                                    demonstrate that GRBV genetic material is respon-
                 Grapevine red blotch virus (GRBV) is the second    sible for red blotch foliar symptoms in red fruited
               virus species discovered in grapevines that carries   grapevine varieties.  The work was done using
               DNA instead of RNA as its genetic material.  Both    sophisticated recombinant DNA molecular tech-
               its molecular and structural characterization has    niques to introduce the virus genetic material into

               Page 38                       The Grapevine • November - December 2020                         877-892-5332





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