Page 42 - Grapevine July-Aug 2020

Around The Vineyard

tive propagated plant
material. The testing
program is known as
Protocol 2010. The
maintenance and
testing of the scion
and rootstock mother
blocks are performed
by UC Davis FPS per-
sonnel. Shortly after
the update of the
California Grapevine
R&C Program, GRBV,
a virus of significant
importance for the vine-
yard industry, was dis-
covered. Consequently,
the California Grapevine
R&C Program was
revised again to include
the testing of foun-
dation and nursery
increase blocks for the
presence of GRBV.

The California
Grapevine R&C Program
rules can be found Newly planted vineyard
in CDFA’s website:
https://www.cdfa.
ca.gov/plant/pe/nsc/nursery/regcert.html

The testing of the foundation mother plants
includes a list of well characterized viruses, Xylella
fastidiosa, and phytoplasmas using biological,
serological, and molecular testing techniques
(https://fps.ucdavis.edu/fgr2010.cfm). The nurs-
ery increase blocks are inspected and tested by
CDFA personnel. The nursery increase blocks are
only tested for GFLV, Tomato ring spot (ToRSV),
and Grapevine leafroll (GLRaV)-1and -3 using the
Enzyme linked Immuno assay (ELISA). The updated
Grapevine R&C added the testing for the detection
of GRBV using the polymerase chain reaction (PCR)
to vines in the foundation and nursery increase
blocks.

Unfortunately, other insect vectored viruses such
as GLRaV-4, Grapevine virus A (GVA), GVB are not

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