Page 21 - Grapevine July-Aug 2020
P. 21

In The Winery

                  liters or 0.048 grams in 400 milliliters.  This is    any filter pad flavor.  This same filtered wine
                  the same as the above calculation using 0.96          can then be moved forward to the protein test-
                  milliliters of a 5% solution in 400 milliliters.      ing below.  Use a new filter pad for each sample
                                                                        to eliminate sloughing of the proteins from one
               5.  When adding the bentonite slurry to the wine –       sample to the next.
                  make sure to be mixing the sample well yet do
                  not use shearing force mixers such as a blender.                Simple Protein Test
                  Use the amount of agitation one can expect to
                  have in the wine tank while adding the fining       Using the clean, dry 20x150 mm screw cap test
                  agents while mixing in the cellar.  Always try    tubes above fill each one about half full with the
                  and mimic the actual cellar experience as close-  varying filtered fining trial levels created above.
                  ly as possible in the lab.  If your lab has a mag-  Label each test tube respective to its “pounds per
                  netic stir bar assembly these work very well.     thousands” contents.  For each wine have a control
                                                                    sample that will go through the heat treatment
               6.  Continue to mix the samples thoroughly after     described and one that will remain with no heating.
                  the addition of the agent or agents.  Perhaps a
                  minute or so on a magnetic stir bar mixer.          Collect each sample after filtering in the lab in its
                                                                    respective test tube labeled 0,1,2…5.
               7.  Discontinue mixing and transfer the wine into a
                  labeled 375-ml wine bottle and place the screw      Heat the test tubes in a crock pot with water to
                  cap on top.  The label should reflect the addi-   roughly 70 degrees C ( near 160 degrees F) for 8
                  tion rate of that sample such a 0, 1,2,3,4,5.     hours.  (See photo bellow)  This is a great place to

               8.  Place, in a dark area, in the cellar or lab at or
                  near the exact wine tank temperature if possi-
                  ble.

               9.  Allow to settle overnight or several days.

               10. Decant into a labeled beaker at least 250 mils of
                  the 400-ml samples from each fining trial.  One
                  portion, approximately 50 milliliters per person,
                  may be transferred into pre-determined labeled
                  clean wine glass for visual, sensory and palate
                  evaluation.   The other portion should move
                  forward for further lab testing for the protein
                  stability examination.

               11. The above analysis will allow one to taste differ-
                  ent fining agent levels to help understand the
                  rate of bentonite added and the expected sen-
                  sory changes, if any, found at different levels.
                  Remember to incorporate an unfined sample in
                  your tastings and lab work as a reference point
                  to determine if a fining should be performed, at
                  all, on a certain wine.


               12. If the wines are not settled enough for visual
                  examination one can employ the 0.45 micron
                  filter listed above to filter out suspended par-  use the test tube holder or coffee cup.
                  ticles.  If sensory is to be done after filtrations     Remove the test tubes from the heat source and
                  make sure to treat the filter pads prior to use   allow to cool to room temperature.  Visually
                  with a light citric acid solution.  This will remove

               877-892-5332                       The Grapevine • July - August 2020                              Page 19





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